FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7276669
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG206970.2844295927161178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT15700.090.489049
ACGTATA14550.081.671759
ACGTTAT11200.075.868568
CCGCATA12850.075.4278269
GACGTAT8150.074.937668
GCGCATA20400.073.220769
GGCGATA21450.072.7314769
GCGGATT27150.072.622059
ACCGATA12800.072.610589
TCGCATA15100.072.5418859
CGTTATT21850.071.096189
CGTGATT18250.070.933859
GTCGATA16600.070.3860249
GTACGAT10050.069.357468
GATCGAT8550.069.102518
GCGTATA21650.068.686599
GCGAATT19200.068.461419
CGCGATA17250.067.3489469
TGCGATA20500.066.062989
ACGGATT29300.065.7070859
Produced by FastQC (version 0.11.5)