FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5709031
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATACCGCCTAAGAACACAGCAAAACGCAATACTGTACATT298750.5232937078113606No Hit
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG199530.34949889044217836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC61800.0103.894344
CCGCCTA65300.0101.584887
CGCCTAA65050.0101.304918
GATACCG62850.0100.962593
GCCTAAG61200.096.6557549
TACCGCC66600.095.940085
TGATACC72700.087.20662
ACGTTAT7050.084.170488
TACATTA58300.082.61894145
ACCGCCT81700.078.9740756
CGCGATT12400.078.583629
ACGTATA10250.074.3472759
GCGCATA14350.073.563769
GTGATAC86650.072.8435751
ACCGATA10700.071.80439
CGTGATT10800.071.139449
CGTTATT15550.069.09199
GCGGATT21550.068.4059459
CCGCATA11600.067.310249
GTACGAT6100.066.559318
Produced by FastQC (version 0.11.5)