FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6143952
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN624831.0169838566447134No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA298720.48620171511756605No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG115460.1879246452446243No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG97740.15908327408807882No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT93980.15296343461016623No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA76630.12472428170011746No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG71680.11666757813212084No Hit
CACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGGAAC63930.10405354729333824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGG108350.088.382894
ATGTTTG105550.087.2161563
GCTAGCC50850.086.669231
CGGTTTA7150.085.046111
GGAGCTT109200.084.665899
TAGTCGA13650.083.135214
GTTTGGA117550.078.313415
CGATACT7500.077.9613347
CGACGTA7450.076.9379355
TTGGAGC122550.075.2998667
GTTGCGA8950.074.3937456
TAGCCCT60300.072.875663
GCCTAAT12550.072.429845
GGACGTA6000.071.40725
CGTGAGT3950.071.05839145
TCGTGTT29650.070.687964
GCTAGTC6750.070.334453
CGCGAGA6850.069.309932
AGATGTT135000.068.508161
CGTACCC9250.068.242661
Produced by FastQC (version 0.11.5)