FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6143952
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG159440.2595072357336125No Hit
ATACAGAACCACTCCAAAGCTCCAAACATCTCAAAACGCAATACTGTACATT151970.24734893762190852No Hit
ATACAGAACCACTCCAAACCTCCAAACATCTCAAAACGCAATACTGTACATT88270.1436697422115277No Hit
ATACAGAACCACTCCAAATCTCCAAACATCTCAAAACGCAATACTGTACATT69070.11241949806899533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT11150.083.967019
CAGAACC113350.078.990884
AACCACT114200.076.5696267
CGTGATT11200.076.199659
GAACCAC117950.075.806626
GCGCTAT7900.074.169168
TGCGATA16500.073.725149
ACGTTAT8750.072.787138
ACGTATA12350.072.714059
ACCACTC120750.071.363048
CCGCATA10650.071.164539
GCGAATT14500.071.156289
ACAGAAC127050.070.87073
GACGTAT6150.067.313318
ATACAGA133150.067.286211
GCGGATT19600.065.9638759
AGCGATA16350.065.442189
ACCGATA11750.065.044359
TCGCATA10400.064.9140559
GGCGATA14600.064.561729
Produced by FastQC (version 0.11.5)