FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5373403
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA226690.4218741828967602No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN199050.370435643855486No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG104760.19496025144587145No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC104330.19416001368220473No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC103810.19319228429358454No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG103750.1930806232102822No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG103730.19304340284918142No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT97290.18105844657473114No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG79430.147820664111737No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC68290.12708892297860405No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG66320.12342271741017749No Hit
CGTCTCCTCGCCGCGATCCTGGAGCGTGAGGTCGCCGCCAGGAACGCCTC54060.10060663605540102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)