FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5373403
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG134930.251107166166394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT10200.078.896079
GTACGAT7100.078.039168
ACCGATA10700.075.2093359
GCGCTAT8600.074.3986368
TCGCATA9950.074.2499
GCGAATT12600.072.7679149
GGCGATA12400.069.685979
GTCGATA12250.068.385399
CCGCATA11600.065.962299
CGCTATT13150.065.209869
CGTGATT11000.064.762989
AGCGATA16450.064.558839
CGTTATA11550.063.963439
ACGTTAT7400.063.287558
ACGTATA9700.062.562179
AGCGATT21050.061.1052069
ACGGATT20850.060.7422529
GCGCATT14350.060.676119
TGCGATA15800.060.5350079
TCCGATT13050.060.1495179
Produced by FastQC (version 0.11.5)