FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453044
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG158340.2453725714561996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA13750.075.2341469
CGCGATT10650.073.0007869
CGTGATT11350.071.895169
ACGTTAT10300.071.114158
ACGTATA14150.070.382879
GACGTAT7150.070.0933768
TACATTA69850.069.18784145
GCGCATA17850.066.592499
GCGAATT10750.066.344719
GCGCTAT8150.066.223198
ACGCATT16950.064.821379
CGTTATA14950.063.608089
GCGCATT18250.062.3163769
CGTTATT20200.061.390019
CGGTATA17100.060.8710379
GTACGAT7600.060.025148
CGCTATT16700.059.251059
CGTAATT18250.059.1477479
ATCGATT16300.059.1283079
CCGCTAA9100.057.8976368
Produced by FastQC (version 0.11.5)