FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5398560
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA197600.36602353220117956No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN149380.27670341720755165No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG140110.25953217154204083No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC138860.25721673927862243No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC120550.22330028748406983No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC116350.21552043507898402No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG113770.21074138288728847No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG113020.20935212352923743No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT109360.20257253786194837No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG103370.19147698645564742No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG90540.16771138970392105No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA67260.1245887792300169No Hit
GAACAGGTCGGCCAGGAACTCGTCGGTGGTCTTCTGGATCACCTGATGCG63390.11742020094247356No Hit
GAGGAACGGACTAAGGCCATCGAGGCATTCCGGGAGGGCAAGAAGGATGT56660.10495391363622893No Hit
CGTCTCCTCGCCGCGATCCTGGAGCGTGAGGTCGCCGCCAGGAACGCCTC56270.10423149877004237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC46250.0106.6882868
TAGTCGA17600.0102.8550954
CGTTGCG41950.098.275525
AGTGTCG42450.096.670897
GGTAGCG53450.092.789316
ACTACGG13550.091.497699
GTTCCGT10400.088.819555
GTAGCGG56550.087.475357
TGAGGTA56850.087.239913
GCTAGCC32000.087.00911
CCACGTT49950.086.384822
CTAGTCG21800.082.75463
GCCCGAT30800.082.547031
ACGGACT14400.081.798776
CGTGAGT3900.081.29384145
GTTGCGC51000.080.9579856
AGTCGAA22400.080.814725
TGTCGAC50600.080.7336049
TCGTGTT28400.080.331374
TCCTCGC91000.079.707675
Produced by FastQC (version 0.11.5)