FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800925
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA247640.3641269386149678No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC172670.25389193381782627No Hit
GAACAGGTCGGCCAGGAACTCGTCGGTGGTCTTCTGGATCACCTGATGCG154430.22707205269871378No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG132660.19506170116565025No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC121490.1786374647566324No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG116210.17087381495899454No Hit
GGCAGCAGTGTCGACGGCAGATAGAGCAATGCTGCGGTGATCAGCAAGGC108320.15927245190911532No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG96150.14137782728084783No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG91470.13449641041475976No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT86350.12696802273220187No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG84530.12429191617316761No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT77570.11405801416719048No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA75690.11129368431500127No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA69140.10166264147891647No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG68060.10007462220212693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC85300.0103.1827248
ACTACGG28100.097.4462669
TAGTCGA40150.096.1109544
AGTGTCG75750.094.283137
GGTAGCG93900.093.5535056
TGTCGAC77050.092.461599
GTAGCGG95150.092.3765957
AGTCGAA42100.092.222465
GCCCGAT51100.090.264971
CTAGTCG43000.089.60233
TGAGGTA98500.089.244033
CCGATGT53950.086.1388243
CGATGTG54700.084.741654
GCTAGCC42900.083.195481
ACTAGTC46350.082.872252
TGACCGA56200.082.2598349
CGGCTTA19700.081.833727
CGTTGCG58550.081.396665
GAGGTAG111850.078.804764
ACGGGAG59400.078.2274559
Produced by FastQC (version 0.11.5)