FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800925
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG213500.3139278848097869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT13900.079.95949
ACCGATA13650.073.3761449
CGTGATT15300.070.5311369
GACGTAT9100.067.4582068
ACGTATA17650.066.265849
GCGAATT17100.065.3741159
ACGTTAT10600.065.227478
AGCGATA17350.065.177019
GTACGAT10950.064.322818
GCGTATT22600.064.04659
CCGCATA16900.063.853639
TCCGATT13700.063.675019
ACGGATT27250.063.5512549
CGATATA17350.063.3148089
GCGGATT22500.062.8951879
CGGTATA17850.062.6273049
CGTTATA17800.062.4401979
CGTTATT18550.062.0057459
CGTAATT24550.061.8546269
GTCGATA16500.061.4853369
Produced by FastQC (version 0.11.5)