FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470224
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC312430.6989135220069509No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG304010.6800777768630833No Hit
GAACAGGTCGGCCAGGAACTCGTCGGTGGTCTTCTGGATCACCTGATGCG281410.629521026239401No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG263920.5903954701151441No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN204420.45729252046429886No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA177000.39595331240671605No Hit
GGCAGCAGTGTCGACGGCAGATAGAGCAATGCTGCGGTGATCAGCAAGGC166480.37241981609870106No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC162620.3637849020541253No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG81870.1831451846708353No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG75160.16813475118920215No Hit
CGTCTCCTCGCCGCGATCCTGGAGCGTGAGGTCGCCGCCAGGAACGCCTC72650.162519820035864No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC72370.16189345321397766No Hit
GCCCGATGTGACCGAGCCCAGGCTGGATGCACCCGAAGCCCGCGCGCGCA68930.15419808940223131No Hit
TCCACGTTGCGCTGCTTGGCGGTCATGTCTCCCGCGCTGACAACCTCGTT65680.1469277602196221No Hit
GCGCTTCGGCTTACGGGTCGGCGTCCAGCGCGGCGTTGAGTCGGTCGTAG60690.13576500864386215No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG52310.11701874447454982No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT49910.1116498860012384No Hit
TCCACGTTGCGCTGCTTGGCGGTGGGGTCGTCGACCACGGCCATCTCGGT48660.10885360554638873No Hit
TGTCCATGGAGGCCTCGTCGGCGTTGCGCGCCACCTCCTGCACGGTCGCT47790.10690739434981333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTCA50400.0123.71303145
TAGCGGC70500.0108.5902258
AGTGTCG77100.0106.455237
AATTGGT37600.0106.088776145
CCACGTT70850.0102.018182
ACTACGG22050.0101.2646949
TAGTCGA17500.0100.627354
TGAGGTA78350.098.282653
GTAGCGG78350.097.7887047
CACGTTG73150.097.216783
ACGTTGC73700.097.0740664
CGGCTTA19450.096.824237
GGTAGCG79500.096.5533146
TCCACGT73800.095.3777851
TGTCGAC85700.094.4124459
CGTTGCG76150.093.9508745
GCCCGAT36250.092.263241
GTTCCGT12700.091.795645
CGATGTG37500.089.664694
CCGATGT38050.088.207373
Produced by FastQC (version 0.11.5)