FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5148188
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA264440.5136564554363594No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC146420.2844107480146413No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG127010.2467081621727878No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG99020.19233951829264975No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG94360.18328778980099406No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT79720.15485059986154354No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN78920.15329665505610907No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN74520.14474995862621956No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT65360.12695729060399505No Hit
CCCATCTCCAGAGCCCTGATCGGCGCACTGCGAGCAGTAGCCCAAACAAT64400.1250925568374737No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA63850.12402421978373751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC45450.0100.247068
TAGTCGA27600.098.194024
CTAGTCG29150.093.169213
GCTAGCC41250.089.020111
GGTAGCG51500.088.7128456
CGTTGCG20000.088.3245855
TTAGGCG16350.084.796849
ACTACGG11650.083.946659
GCCCGAT34900.083.613021
GTAGCGG54650.083.476347
TGACCGA35950.082.251659
CGATGTG36250.081.7471164
TGTCGAC32100.081.543019
AGTGTCG32550.081.299387
TGAGGTA56200.081.0882343
AGTCGAA34150.079.528815
CCGATGT37200.079.349363
GTGTCGA33850.077.667258
ACTAGTC35150.076.937362
GACGGGA47300.076.7447368
Produced by FastQC (version 0.11.5)