FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5148188
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG167020.3244248267545785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTA56050.082.93075145
GCGCTAT6800.073.9610068
CCGCATA9850.073.0359
CGCGATT9650.072.569519
TCGCATA10200.071.153059
GCGAATT11750.069.352329
ATCGATT10350.068.891649
ACGTTAT7000.067.30058
AGCGATA12600.066.189579
GCGCATA14100.065.9208159
ATACCGC12950.065.8684544
CGTGTAT6150.065.2153858
CGCCTAA15650.065.08648
CGCGTAT5100.064.910938
GCGTATA12650.064.418169
ACCGATA9000.063.6632589
CCGTTAT7300.063.662648
CGTTATA13200.063.1809589
CCGCCTA14700.062.793437
CGGTATA14000.062.753789
Produced by FastQC (version 0.11.5)