FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484318
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN238410.5316527507638843No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA186880.41674118561618506No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC96580.21537277240374123No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG88900.1982464223099254No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT82700.18442046259877198No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG73830.16464042023781544No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG51840.11560286313325684No Hit
CATTTGCTTTGCCATCTCCGCATTGTCCACTCACTTGCTCTGCCCTTCTG51250.11428716696719546No Hit
CAGTACTGCATGAGTATATGAGCTTCCCCAAGCTGGGGCCAAAACAGGGC48990.10924738165312986No Hit
CAATCTCCTAACAAACAAAACTTAAAAGGCACCATGCACTTAATTGGCCG48290.10768638620187061No Hit
GCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGGA47270.1054117928300357No Hit
CAAGGAGCATCAGGGAGAGAAGGATGTGACTCACGGCCTCAAGCTCCTGG46210.10304799971812882No Hit
TGGAGGCCACTAACCCACTTGTGATGCAGTTGCTGCAGGGTAGCTTGCCC46200.10302569978311084No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCGTAAAACGTGCCTTT46000.10257970108275105No Hit
CTCCATGCCCATCCTATCTGTGGATCCCGGGTGGAACCTCAGCTCCAGAA45880.10231210186253518No Hit
GCAAGTTCGAGATCATCAGGAAGGACCATGGTTCCCAGAGCTTCAGATCT45800.10213370238239126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)