FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238936
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA143150.4419661271479276No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN135540.41847075706343073No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG74690.23060041939698717No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG73020.22544440519973227No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA50900.1571503728384877No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC48950.15112987721893856No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT44160.13634107003040505No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG43300.13368587709050134No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN43200.13337713372539625No Hit
ATCAAACGGATCTACCACGATGGGTTCAGTACCTTTAATTTCACATCGGC41460.12800499917256777No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT40520.12510281154057998No Hit
GGAGAGCTAGAGGAATAATTAAAGCTGGTGGAACTCAGTTGGAGTTTAGA39440.1217683831974451No Hit
CGTACCCAGAAGACAATGGCCTAGCTATTGGTTTACCCACCTTGGTTAAG38920.12016291769889864No Hit
AGCGGCATTGGGACAGACAACAACAGCACTTCTGACCAAGCGGAGAAGAG37490.11574788757789595No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG35730.11031400435204647No Hit
GGCAAGATTGAAGGTAGGAGATAAGAGGTGGGGCAGAAGAAGGTCAGCAT35210.10870853885350004No Hit
AATCTACTATTCGGTGTAGACTCTAATCGGGCAATTTTAATTGGAGGGTC35100.10836892115188444No Hit
CAGTCTAGCCTTTGAATTGATGCGCTTGACCTCTGTGCTCTTCTGGCTGC34750.10728831937401664No Hit
GCAAGTTCGAGATCATCAGGAAGGACCATGGTTCCCAGAGCTTCAGATCT33020.10194705915769871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)