FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299415
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG159610.30118418731124097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTA52250.099.751145
CGTGATT10250.085.962149
CGCGATT9150.083.915429
GTACGAT8750.079.118018
TCGCATA6300.074.924499
ACCGATA10300.073.324159
AGCGATA11300.071.290989
CGTTATT11650.070.7698759
GCGCATA12150.069.411529
GCGAATT12650.067.663039
CCGTTAT5900.066.134868
CCGCATA11250.062.656859
ACGTTAT7200.060.31258
GCAATAA59800.060.04913145
CGTTATA11850.060.0154579
CGGTATA11850.060.0154579
CACGATT12150.059.5695889
GCGCATT17900.058.7173549
TCGCATT10100.057.328369
ACGGATT17700.057.2473879
Produced by FastQC (version 0.11.5)