FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002978
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA33660.33560058146838717No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG25090.25015503829595465No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT23390.23320551397936945No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC18130.1807616916821705No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG16480.16431068278666133No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA13560.13519738219582086No Hit
AATCTACTATTCGGTGTAGACTCTAATCGGGCAATTTTAATTGGAGGGTC11310.11276418824739926No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA11020.10987279880515825No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT10770.10738022169977807No Hit
TCCCCGACGTACATGATCTTCCCCTGGTGCCGCACCATGCCCACCGTGAT10750.10718081553134766No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG10730.10698140936291722No Hit
ATGGAGCCAGAGGAGGAGGATGAACTAGGCTCAGGGCGGGATGTGGATTC10710.10678200319448682No Hit
GGGAATCTGCAGAGAAAAATCAGAGACCAAGGAAGCAGACTAGTGCTCCG10590.10558556618390433No Hit
GCATTTTATGGGAAGCTTTCTAAACTCCAACTGAGTTCCACCAGCTTTAA10460.10428942608910666No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG10430.10399031683646102No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA10350.10319269216273937No Hit
CCGCGTTGGAGTTGGAATCCACATCCCGCCCTGAGCCTAGTTCATCCTCC10330.10299328599430894No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG10290.10259447365744813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA200.0020129643220.85655145
CTAGCGT1100.0160.62294145
CGCCAAC756.892151E-9103.2634135-139
CGCACGT1600.0102.684851
CTAGTCG3750.092.318573
CACGTCG1800.091.275433
TAGTCGA3950.087.644214
CGCAATC950.086.471463
TCGTGTT5300.085.247814
CGACGTA1850.082.4650655
TGACCGA4800.081.8993159
CGTCGGA3450.081.637655
GTAGCGC851.168848E-681.267784140-144
CGTACCC1750.080.47141
TAGCGGC2800.079.629188
CGTTGCG1950.078.236095
CGTGTTC5850.078.2360845
GCCCGAT4800.078.236081
CGATACT1250.075.10297
AACGGAC1800.074.976245
Produced by FastQC (version 0.11.5)