FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881473
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA192080.4948636767536448No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC136400.35141298161806095No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG82720.21311496949740472No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG80460.20729243769053657No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN71390.18392502021784No Hit
CCCATCTCCAGAGCCCTGATCGGCGCACTGCGAGCAGTAGCCCAAACAAT69640.17941642257977836No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG69060.17792214450544935No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT67740.17452137371559714No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA65220.16802899311678837No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT51340.13226937299319097No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA47190.12157755573721626No Hit
GGAGAGCTAGAGGAATAATTAAAGCTGGTGGAACTCAGTTGGAGTTTAGA42080.10841245063407629No Hit
CACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGGAAC40980.10557847497586612No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCAGAGCTGCAAG39570.10194583345034217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA22400.0114.290144
TAGCGGC29800.0111.7840358
CGTGAGT3100.0100.50447145
AGTCGAA25650.0100.29825
TTAGGCG14500.099.9414149
GCCCGAT32100.097.151161
TGACCGA32300.095.5573359
CTAGTCG27100.094.237073
CCGATGT33800.093.193083
GGTAGCG36050.092.424236
CGATGTG34450.091.616864
CGTTGCG11750.091.317685
GTAGCGG37450.089.284667
CGAAGAC49550.088.118498
GCTAGCC31950.087.787621
ACGGGAG34400.087.717889
CTTCTCG46350.087.047813
ACTAGTC29500.086.570322
CGGTTTA4000.086.277851
CGACGTA4600.085.936845
Produced by FastQC (version 0.11.5)