FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5672155
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG157110.27698467337370014No Hit
GTGATACCGCCTAAGAACACAGCAAAACGCAATACTGTACATT59330.10459869308931086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT12150.085.171829
GTACGAT6350.084.1476068
CGCCTAA29600.083.404518
CGCGATA12350.082.149529
CCGCCTA26950.081.317187
GCGAATT14850.078.795329
GGCGATA15500.078.545549
GCGCTAT8800.077.629568
TCGCATA10550.077.573559
ACCGATA10600.077.207649
ATACCGC26450.076.4664
TACCGCC27750.073.614445
GATACCG27450.072.453353
CGTGATT13250.070.954459
TACATTA85000.069.48715145
CGTTATA12250.069.016789
CGGTATA13400.068.645949
CGGGATA19650.068.152759
AGCGATT19000.067.6364449
GCGGATT23100.067.6364449
Produced by FastQC (version 0.11.5)