FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4690823
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN254870.543337491096978No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA236400.5039627374556661No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC149450.31860080842956556No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG123300.2628536612871558No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT100910.21512216512965848No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG90500.19292989737621735No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG86440.18427469977016828No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN85450.18216419592041738No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC56000.11938203594550466No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA55570.11846535245520881No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT54540.11626957572263971No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT52150.1111745209742512No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA50750.1081899700756136No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG50530.1077209692201134No Hit
GTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGGTAT46910.10000377332506472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA20850.0105.820224
TAGCGGC31000.0101.205158
AGTCGAA23450.093.8345645
TTAGGCG18450.091.613019
GCTAGCC41050.090.157781
CGTTGCG17250.088.020265
ACGGGAG38850.087.167339
TGACCGA29500.087.0511559
CTAGTCG25450.086.2274863
GCCCGAT29600.085.960191
GACGGGA39600.085.366668
CGATACT5600.084.724817
TCGTGTT30250.084.701324
CCGATGT30700.084.232543
TGTCGAC23800.083.229669
CGATGTG31450.082.035234
CGAAGAC47350.081.6650548
CGTGAGT3200.081.5774145
GCTCATG77150.080.643875
CACGGGT19500.079.9889149
Produced by FastQC (version 0.11.5)