FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171164
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN129460.5962700192154992No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA107580.4954945826294099No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG58390.2689340832843581No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN55170.25410332890560083No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG35210.16217107505467113No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN32210.1483536020309843No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA28840.13283197400104277No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCAGAGCTGCAAG27150.12504813086436584No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT24840.11440867663612699No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT24300.11192153149186335No Hit
TGCAGATCCCGTACCAGCTTTGGATCTCGGACAGCAACTCCAGCTCAAAG23650.10892774567006454No Hit
GAGCTGCGGTACTCGTCCCAGTACGAGGAGCGCCTGGACCCCTTCTCCTC23170.10671694998627465No Hit
GAGGAACGGACTAAGGCCATCGAGGCATTCCGGGAGGGCAAGAAGGATGT23120.10648665876921319No Hit
CACCCCTCACCTGTAGCGATTCCATCAAAGAGAGACAGCACCCGGATGGG23080.10630242579556404No Hit
GCTCACGAACCCGCTTGCTGAAGGAGGAGAAGGGGTCCAGGCGCTCCTCG22720.10464432903272163No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT22610.10413768835518644No Hit
CCCAGTAGCTTTCCTGTTCTCGGCATTTGCCTTAAGAGGACCCCCCACAA22460.1034468147040021No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG22190.10220324213187028No Hit
TCCCCGACGTACATGATCTTCCCCTGGTGCCGCACCATGCCCACCGTGAT22180.10215718388845799No Hit
GGGCATTCAGGTGGACCGCTACATTGCCTCGGAGGTGTGTGAGGACTCCA22100.10178871794115968No Hit
CTTGATGGCTGTTTTGGGTTCTTCGGATGGGGGTGGCAGCTCTGCTCTTG22100.10178871794115968No Hit
AATCTACTATTCGGTGTAGACTCTAATCGGGCAATTTTAATTGGAGGGTC21950.10109784428997534No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA21910.10091361131632616No Hit
AGCGGCATTGGGACAGACAACAACAGCACTTCTGACCAAGCGGAGAAGAG21880.1007754365860893No Hit
GTTAAGTTTCTTGATGCCTTGATTGACACGTACCTGCCTGGAATTGATGA21850.10063726185585245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)