FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171164
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG60060.27662580993421043No Hit
GTGATACCGCCTAAGAACACAGCAAAACGCAATACTGTACATT53350.2457207286045642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32540.14987352406358986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA16350.095.910417
GATACCG15700.093.840023
ATACCGC15850.093.747644
CGCCTAA16600.089.897118
GATCGAT2300.087.975958
CGTGATT5200.087.548959
TACATTA27000.085.66659145
TACCGCC17400.085.031225
CGCGATT5050.083.889049
GTACGAT2200.083.352228
CGCGATA3750.079.248029
CGAGCAT3450.078.811788
ACCGATA4100.078.65179
CCGCATA6150.076.081389
CGCTATT6050.075.248699
GCGCATA5800.075.221669
CGGTATA6500.074.902999
CGAGCGA1100.074.727857
CGACGAT2050.074.028548
ACGCGAT2650.073.9703458
Produced by FastQC (version 0.11.5)