FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011046
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA152840.507597691964852No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG59030.19604482960406452No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN56570.18787491124346822No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG47570.1579849660217745No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA40860.1357003513064895No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG37630.12497318207692609No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT37320.12394363951928997No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC37020.12294730801190018No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG34720.11530876645524511No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA30670.10185829110548296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT1500.0127.065575145
TAGTCGA8750.0103.271894
CGGTTTA3500.097.644891
CGACGTA5750.091.672515
CGTCCGT1251.2732926E-1191.24042140-144
GTTCCGT3900.087.630035
CGGGTTA4050.087.2449655
CGTTGCG5400.086.88745
CGGATCT3850.085.754727
GCTAGCC24150.083.709331
CTAGTCG10800.083.669353
CGATACT3350.082.992797
CGCACGT5700.080.281931
TCGTGTT13800.080.171474
AGTCGAA11400.079.26575
CGTCGGA9650.078.033595
ACCGGTT2900.077.899056
GCTAGAC5250.077.2332462
GCTAGTC2800.076.543673
TACGGTG3900.075.747992
Produced by FastQC (version 0.11.5)