FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723315
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG53980.31323350635258207No Hit
GTGATACCGCCTAAGAACACAGCAAAACGCAATACTGTACATT42020.24383238119554465No Hit
ATACAGAACCACTCCAAACCTACAAACATCTCAAAACGCAATACTGTACATT22650.13143273284338614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19530.11332809149807203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA12650.0109.720967
CGCCTAA12200.0107.541948
CGCGATT3150.0107.413059
GATACCG12700.0102.226493
ATACCGC13350.0101.384494
TACCGCC13450.0100.1143655
TCGCATA3350.092.7553949
GTACGAT2150.086.7180258
GCGCTAT2550.086.655058
CGCGATA3050.083.767089
ACGCATT3800.078.1369259
GCGAATT5200.075.690799
GACGTAT2300.075.057888
ACGTTAT3500.074.972098
GCGCATT4900.074.688079
ACGGATT7050.074.4382259
GCGGATT7850.073.009599
ACCGCCT18350.072.6282046
CCGCATA4850.072.610599
GCGCATA4700.071.989599
Produced by FastQC (version 0.11.5)