FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2513760
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG72640.2889695118070142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27980.11130736426707402No Hit
GGGGGGGGGGGGGCCAAGACTTGGGGGGGGGGGGGGGGGGGGGGGGGGGG26900.10701101139329132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT7850.078.930519
ACCGATA5300.078.813389
CGTGATT6950.077.131279
TCGCATT7200.073.486189
TCGCATA5750.071.4346169
GCGGATT10450.071.2840049
TCCGATT6650.071.188839
ACGGATT11550.071.125389
AGCGATA7050.071.099739
GACGTAT2650.070.933458
CGTTATA5500.070.884279
GCGCATT8700.070.418699
GCGCATA7550.070.079549
CGGGATT15300.070.073519
GTACGAT3300.069.619878
CGCGATA7000.069.6184849
ACGTATA5300.069.6184849
TAACGAT5450.068.9811558
CCGCATA7400.068.67779
AGCGATT10100.068.239919
Produced by FastQC (version 0.11.5)