FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630884
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN111430.30689496001524696No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA111070.30590346593281414No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN96150.2648115445164318No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA61500.1693802390822731No Hit
GAGGAACGGACTAAGGCCATCGAGGCATTCCGGGAGGGCAAGAAGGATGT55110.15178121911909057No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA54780.15087234954352713No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG54300.14955035743361672No Hit
GAGCTGCGGTACTCGTCCCAGTACGAGGAGCGCCTGGACCCCTTCTCCTC53490.14731949574814288No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT52910.14572208861533445No Hit
AGGCCTATGCTGACCGTACACCCCTCCCAGCCGCCATCCTGAATGCCTCC52050.1433535194184116No Hit
TGCAGATCCCGTACCAGCTTTGGATCTCGGACAGCAACTCCAGCTCAAAG51820.1427200648657462No Hit
GCTCACGAACCCGCTTGCTGAAGGAGGAGAAGGGGTCCAGGCGCTCCTCG50160.1381481754856393No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN49590.13657830985512068No Hit
CAATGACAGCAGCTGACTTGACTTTGCTAATGCCAAACAGCAAGTAAAAT48630.13393432563529983No Hit
CCCCCGAGCAGTACGAGGTCTACATGTACCAGGAGGTGGACACCATCGAG48430.13338349558950383No Hit
CAGTCTAGCCTTTGAATTGATGCGCTTGACCTCTGTGCTCTTCTGGCTGC47910.1319513374704342No Hit
CTGAGTGGAGTCACAGCGGAGATAGTGCCCTGGCCCTGGGCTTGTGGGGC47630.13118017540631977No Hit
TCCCCGACGTACATGATCTTCCCCTGGTGCCGCACCATGCCCACCGTGAT45610.12561679194378006No Hit
GGCAGGCCTGATTCAATGACTCTGTGGGGCTGGGTCTTAGGTACCCCATC45120.12426725833157987No Hit
GGAGATGGAGAGAGGAGAGCAGGACGGGAGGAGCTGGCAGTGGAAGGCCC45070.12412955082013086No Hit
GCAAGTTCGAGATCATCAGGAAGGACCATGGTTCCCAGAGCTTCAGATCT45050.12407446781555125No Hit
CTATTGCTGGCACCATCTGACGTGGCAGGCTGGGGGATTTTTATCAAAGA44890.12363380377891445No Hit
CACCCCTCACCTGTAGCGATTCCATCAAAGAGAGACAGCACCCGGATGGG44710.12313805673769802No Hit
GAAACTGGCTGAGCACATAGAGCATGAACAACAGAAACTACCTGCTGCCA44630.12291772471937965No Hit
CCCAGTAGCTTTCCTGTTCTCGGCATTTGCCTTAAGAGGACCCCCCACAA44600.12283510021251023No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC44060.121347859088861No Hit
GTGTGCCACGACAGCGATGAGAGTGACACTGCCAAGGCCGTGGAGGTGCA43320.11930978791941578No Hit
TGCTGGCTGTGGTTTTACTCCCGATGCCTTTAACACTCATATGTCTGGGA42760.11776746379118695No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT42690.11757467327515835No Hit
GGAGAGCTAGAGGAATAATTAAAGCTGGTGGAACTCAGTTGGAGTTTAGA42660.11749204876828893No Hit
CAAACAGACGCTGGGCTATCGAAGCCCCGACGCCGGGCCACCGCACGCTT42570.11724417524768074No Hit
TCCCGGGCTCGATTCACAGGCTCATGCTCCAGGTGCTGCAGGCTGTTGGC42500.11705138473165212No Hit
CTTGGATGTCCTTTTCCCTGTACGTTCCAAAGCCTTCATAGCTCCTCCTT41870.11531627008739469No Hit
CACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGCTTGCGCTTC41760.1150133135622069No Hit
TCCAGGCTGAGGTTTCGACTGAAGCAGGGGTTGTCCTGCGCCCATGTGCC41450.11415952699122309No Hit
GGCAAGATTGAAGGTAGGAGATAAGAGGTGGGGCAGAAGAAGGTCAGCAT41420.11407690248435368No Hit
GCTTGGACATGGGCACGATCGGGGAGGATGGCAGGGGGCTGTTCTGTGGT41370.11393919497290467No Hit
AGCATCTGTGGTGTCATGCTAATAGCCAGGCACTGATGGCCTGTGTGTTC41320.11380148746145567No Hit
CGAAGTGCGGAAGATGTGCAACTACACCAAGATCCTGTCCACCAAGAAGA40970.11283753488131264No Hit
TGGAGGCCACTAACCCACTTGTGATGCAGTTGCTGCAGGGTAGCTTGCCC40960.11280999337902285No Hit
GGGAATCTGCAGAGAAAAATCAGAGACCAAGGAAGCAGACTAGTGCTCCG40890.11261720286299426No Hit
TTGTCCCATGCCTATTGTCAGATAAGCACTGCCTCCCTTTGTCCCTGGCC40850.11250703685383504No Hit
GCCAGCGGATGCAGCGTATCTGCAGGGTGTAGGCCGTGGCTGGGAGGAGC40620.11187358230116964No Hit
CTCCCAGTAAGTTCTAAGGGTTAGCCTGAAGGGGAAACTGAGGCCCATCA40580.11176341629201042No Hit
AAAATGGAAAGATCGGAGGGTGTGTCTGACTCAGCCTCCACCTGCCCCCT40340.11110242023705522No Hit
CGGTTTATAGAGGATGAGGATTATGACCCTCCAATTAAAATTGCCCGATT40070.1103587996752306No Hit
GGGCATTCAGGTGGACCGCTACATTGCCTCGGAGGTGTGTGAGGACTCCA39940.1100007601454632No Hit
ATGGAGCCAGAGGAGGAGGATGAACTAGGCTCAGGGCGGGATGTGGATTC39920.1099456771408836No Hit
GGGTCCTTTCCTGACTGTCCCTCCCTGTGCAGGAGCGGCAGAGGAAGTAC39810.10964272061569579No Hit
CTGAGCCGGTCTCCTTGTGACTAACCTGCTCTCTCCCTCTCACCCTCCTA39780.10956009610882639No Hit
CCCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGCAC39570.10898172456074058No Hit
AGGGACGAGATGTTGCCGGAAGTGACAGGAAGGCCTGAGTACTTGGCTTC39350.10837581151036497No Hit
CTTGATGGCTGTTTTGGGTTCTTCGGATGGGGGTGGCAGCTCTGCTCTTG39250.10810039648746696No Hit
GATCCAGACTCGGAACCGGAAGATGTCCAACAAGTCCAAGAAGAGCAAGA39210.10799023047830776No Hit
GAGTAAGTTTGACATTGCAAGGACCGTACAGTGCCTCAGTGATGCCAGCC39130.10776989845998936No Hit
TAGCTACTGCCTGCCTTCGAAGAACGATGATTCAGAAGAAGAGGTGAGAG39020.10746694193480157No Hit
AATCTACTATTCGGTGTAGACTCTAATCGGGCAATTTTAATTGGAGGGTC38980.10735677592564234No Hit
CCGTTCTTGGCCAGCTTTTCCTTAACCTGCCGGGTGAGCTCGATGGTGTC38730.10666823836839735No Hit
GCACCAGCGATCAGGTCCTTTATGGGCAGCTGCTGGGCAGCCCCACAAGC38610.10633774034091972No Hit
CTCAAAGGGTTGTGACAGGTCCCTCTAAGAGAGGAGAATCCTCACCAGAA38590.10628265733634013No Hit
CCGTCAGTGACACCTGGTACTTGACGCCGTCCTTGTCCTCTCCTCGGGCT38570.10622757433176053No Hit
CAACTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCA38560.10620003282947074No Hit
GGTACAGGCATCCTTCACCTGCTATTGTTGCAAGAACAGTCAGAATTTTA38280.10542887076535631No Hit
GTCCCTGGAGCTCGTGGTCGGACCCAACTAGGGTGGAGACCGCCACCGAG38250.1053462462584869No Hit
CTTGGAGGCAACGTCTGTGGCTACTAGGACATCCTTCTTGCCCTCCCGGA38120.10498820672871952No Hit
GCACTGCTTCTTTATATTGCGACCACCAAACTTGGGCTTATCTAAGCAAT38020.10471279170582151No Hit
GGCTTATGTCTAAGTTTTTCAAGCACAGGGTAGAATATTGGGCTGAGTAA37940.10449245968750309No Hit
AACAGAAACAAGAACTTCAGGCGCCAAGTAGGTGGGGGTTCCACATAAGG37480.10322555058217228No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG37460.10317046757759268No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC37410.10303276006614367No Hit
CAGGCCCATCACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAT36950.10176585096081285No Hit
AGCGGCATTGGGACAGACAACAACAGCACTTCTGACCAAGCGGAGAAGAG36760.10124256241730664No Hit
TAGCGCTGGAAGAGGTCGGTGAACTGCAGCGACACCAGCTCGGCGCGCAG36730.10115993791043724No Hit
CATTTGCTTTGCCATCTCCGCATTGTCCACTCACTTGCTCTGCCCTTCTG36720.10113239640814743No Hit
CAGGCAATCAGTCGGTGAGGATTCAGGTGTGGAACTGGGGGTCACATTTA36710.10110485490585765No Hit
GCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGGA36610.10082943988295964No Hit
ATACTGCCCAATTTTCTCCTGATTGTTCCAGAGTAGACGATGTAAAGCAA36550.10066419086922082No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT36460.10041631734861263No Hit
GTTAAGTTTCTTGATGCCTTGATTGACACGTACCTGCCTGGAATTGATGA36440.10036123434403303No Hit
CACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGGAAC36440.10036123434403303No Hit
GCCAGTTCCTTTCTCTAATGTATCATCTCCCACTAGAGACGGAATGGGAC36440.10036123434403303No Hit
AGGTTACAGCACGGTCACCTTCGACGGGACGCCCAGCTACGGTCACACGC36310.10000319481426562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)