FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066270
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA273580.8922241029002664No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG125910.4106292009509926No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG86860.28327577154001443No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT80320.26194692574365597No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC70170.2288448179710202No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA68890.22467036497112128No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG62340.2033089062607011No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC47460.1547808901368764No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT46580.1519109536994459No Hit
GTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGGTAT38880.12679900987192907No Hit
ATCAAACGGATCTACCACGATGGGTTCAGTACCTTTAATTTCACATCGGC38150.12441826714542424No Hit
AACAGAAACAAGAACTTCAGGCGCCAAGTAGGTGGGGGTTCCACATAAGG36810.12004813666115507No Hit
CAGTTCTATTTCCAAAGATCATTATGGCACTTTCCTTCTGGTTCAGAGCA34940.11394952173161528No Hit
TAATAACTCCCAACTCACTGGTCCTCTAAACCTGCAGCAGGGAGATGGTG33320.10866622965361825No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT31880.10396997002873197No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG31180.10168706604441226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA13000.0114.975824
GCTAGCC38950.0110.700751
CGTGAGT2500.0104.71391145
CTAGTCG14750.0100.4610443
TAGCCCT46650.091.978113
TAGCGGC10200.090.9381948
TCGTGTT24650.090.431774
CGTGTTC24950.089.344435
AGTCGAA16850.088.705395
CGCACGT5450.088.6944961
CGGATCT7100.088.0029757
CGTGTCG2400.087.2616145
GTTATCG609.275778E-487.2616145
CGACGTA3600.085.9014745
CGCGAGA2900.084.42182
CCTGTCA51000.083.991537
CGTCGGA8150.083.793475
CTACGGA1051.0243257E-683.106285145
CGGTTTA2950.083.0220641
CGATACT3000.081.591777
Produced by FastQC (version 0.11.5)