FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338070
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG114860.21517140089957607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT9800.088.359819
ACGTATA10650.079.401979
GCGAATT15200.074.3265159
ACCGATA9050.073.2568749
CCGCATA9900.072.433859
GGCGATA14800.072.2214669
GACGTAT6950.072.030738
GCGTATT16600.070.910749
GCGGATT20500.069.9604959
TACATTA78200.069.71558145
GTACGAT7800.069.38518
TCCGATT12450.067.37889
CGGTAAT14750.066.962518
GCGCATA13950.066.923069
TCGCATT13600.066.158189
CGCGATA12550.065.763819
CGTAATT15500.065.468219
CGGTATA12550.065.224769
CCGGTAT11350.064.372268
ATCGATT13250.064.331789
Produced by FastQC (version 0.11.5)