FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5959875
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG243020.40776022987059296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT9500.079.493749
CGTGATT11550.077.272549
TACATTA66250.076.782814145
ACCGATA12100.070.149519
CCGCATA13600.068.3783349
TCGCATA10500.068.356489
GCGAATT12250.066.2336049
GCGCATA14650.066.033649
GACGTAT6750.065.648098
ACGTTAT6900.065.125478
CGTTATT15000.064.492859
CGGTATA16850.064.449649
CGGTATT20550.063.7791379
GCGGATT18450.062.24339
ACGGATA17850.062.2376149
ACGTATA13500.061.0254949
ACGCATT11800.060.825689
GCGCATT15400.059.9807859
ACGGATT21700.059.8239029
CGACATT11900.059.790079
Produced by FastQC (version 0.11.5)