FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005791396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80123547
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT14409811.7984488380176178TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCGCGTAT2063600.2575522523984117TruSeq Adapter, Index 15 (97% over 37bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT1793750.22387301450845654No Hit
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG1087680.13575035563515428No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTA1014000.1265545570517491TruSeq Adapter, Index 15 (97% over 37bp)
CCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGC890480.11113836485546502No Hit
GCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGT832160.10385960571615731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)