FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005791398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120079997
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCTCGTAT20598301.7153814552477047TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCGCGTAT2807280.2337841497447739TruSeq Adapter, Index 9 (97% over 37bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT2633870.2193429435212261No Hit
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG1651800.13755829790701943No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCTCGTA1548270.12893654552639605TruSeq Adapter, Index 9 (97% over 37bp)
CCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGC1333700.1110676243604503No Hit
GCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGT1202390.1001324142271589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)