FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38981832
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT566958314.544167652254004TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCGCGTAT8096992.0771188999018824TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA3497330.8971692248840434TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCGCGTA1995870.5120000517163996TruSeq Adapter, Index 10 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATG983110.25219697216898374TruSeq Adapter, Index 10 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA961680.24669953941620806No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT944710.2423462293921948Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC842230.2160570596066393No Hit
CCCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCAC697790.17900390109936343No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT682460.1750712998814422No Hit
GCCTTGTTCAATTAAGCACTCTATTCTTAATTTACTGCTAAATCCTCCTT680310.17451976089784593No Hit
CTGGCGAGTAGTTTTGTTGGTTTAACTACTGAGGTTTAGGGCTAAGCATA617270.15834812483928412No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCGCGTATG585820.15028026389319005TruSeq Adapter, Index 10 (97% over 36bp)
GTGGGGTTTAGCTTTAATTAGGTTGGGTCTAAAACACTCTCTACGCCGGT554730.14230475366062836No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG538580.13816179803966114No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT532810.13668162132554468No Hit
CCCCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCA511860.13130732285747884No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT507900.13029146500862246No Hit
GTCTGACTTAGGCTTATGCGGTGGAGAAAATGTTCATGTTACTTATACTA496430.1273490686635764No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCC490540.12583810837828246TruSeq Adapter, Index 10 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCGCGTATGCC456860.11719818606780717TruSeq Adapter, Index 10 (97% over 34bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT451050.11570774816329822TruSeq Adapter, Index 7 (97% over 36bp)
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG421760.10819399149839853No Hit
CCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCACT414790.10640597907250741No Hit
CCCACCAGCGCCTCCATCGAAACTAGCGTTCCGATTTCTACGGCTCCTAC412620.10584930949371493No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC395260.10139595286337492No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)