FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66939518
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTAT893073813.341503295557041TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGGAT28076514.194310153234148TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTTT19554062.9211533910357703TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGGTT7875181.1764620115728948TruSeq Adapter, Index 7 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT7722321.1536264721834417Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGTAT6173250.922213093915615TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGGT4847000.7240864805748976TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTT4841170.7232155451134261TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGGAT2760210.41234387137355843TruSeq Adapter, Index 7 (97% over 36bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC2326330.3475271513009699No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2254230.33675623418740486No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGGT2217850.3313214773969541TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTGT2056550.30722509833429035TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGTTT1879790.2808191717185654TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGTT1876690.2803560670992582TruSeq Adapter, Index 7 (97% over 36bp)
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1845850.27574892308008553No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1394460.20831640885134545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGGGT1297770.19387202638656587TruSeq Adapter, Index 7 (97% over 36bp)
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1243910.18582595709756977No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG1166600.17427672544639478Illumina Single End PCR Primer 1 (96% over 32bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG1119470.167236041347056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCGCGGTT1069950.15983831852509006TruSeq Adapter, Index 7 (97% over 36bp)
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC975010.14565536608733873No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT941500.14064935454121436Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG871760.13023099449267023No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTA811670.12125423430745348TruSeq Adapter, Index 7 (97% over 36bp)
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA803540.12003970509617352No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC788270.11775854137461819No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC736150.10997240822678167No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGGTTG672830.10051312290596415TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)