FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005791414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79035692
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTAT40641075.14211604549499TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCGCGTAT5443690.6887635019378333TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTA2016320.25511511938175985TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTTT1477920.186993997597946TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGGAT1151360.14567595612372192TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCGCGTA1066880.13498711442926317TruSeq Adapter, Index 1 (97% over 38bp)
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG902580.11419903807510157No Hit
CTCCCGTGCAATATCTAGGAAAATCCTTATATTTAAAAATTATTTGTTGT843430.10671507753737387No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTT807340.10214878614588456TruSeq Adapter, Index 1 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)