FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35647937
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT373803710.485984083735337TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCGCGTAT5424041.5215578954821425TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTA1970040.5526378707412998TruSeq Adapter, Index 25 (97% over 37bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1397670.3920759846495465No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1336130.3748127135660052No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1273980.3573783245857958Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1134360.31821196272872676No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCGCGTA1092810.3065563092753446TruSeq Adapter, Index 25 (97% over 37bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT842880.2364456602355418No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG794230.22279830667339878No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATG697010.19552604124047906TruSeq Adapter, Index 25 (97% over 36bp)
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT665550.18670084611067395No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTTT657520.18444826133977962TruSeq Adapter, Index 25 (97% over 37bp)
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC521750.14636190587971473No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA506770.1421596991713714No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC503200.1411582386941494No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCGCGTATG438470.12300010516737617TruSeq Adapter, Index 25 (97% over 36bp)
CCCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCAC414950.11640224790567825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGGAT403870.113294073651443TruSeq Adapter, Index 25 (97% over 37bp)
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC402780.1129883056065769No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC398460.11177645427279563No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA391270.10975950726124768No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG379160.10636239623067108No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC360850.10122605411920473No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)