FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71658473
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGGT32160294.487995439143672TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGTT16792652.3434283898290715TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGGG6290820.8778892064864402TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCGCGGGT4824360.673243483711968TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGAT3799680.5302485304145401TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTGT3494280.4876297043058676TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTTT2981590.4160833848636434TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGG2568020.3583693445435266TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGT2467960.34440588763313446TruSeq Adapter, Index 5 (97% over 37bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC2232240.311510963958163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGGTG1902890.26554989526500233TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCGCGGG1895380.26450186846711066TruSeq Adapter, Index 5 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1838140.2565139784656031No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1663270.23211072331948798No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCGCGGTT1587730.22156905297158647TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT1504630.20997237828386325TruSeq Adapter, Index 5 (97% over 37bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG1472990.20555698975053516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCGCGGGG1334200.18618872886113552TruSeq Adapter, Index 5 (97% over 37bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1263520.17632527558883374No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1193890.16660835069706273No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCGCGGT1070490.14938777721372878TruSeq Adapter, Index 5 (97% over 37bp)
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC994170.1387372572117187No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG743170.10370999672292766No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC740340.1033150678496875No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA722770.10086315961547214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)