FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32912535
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTAT2240820.6808409014984717TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGGAT1684300.5117503103300916TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGGTT1518010.46122548749283515TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTTT1221570.3711564606008015TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGGGT1025300.31152264631089643TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTGT507910.1543211423854164TruSeq Adapter, Index 16 (97% over 37bp)
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG454670.13814493474902495No Hit
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT368320.11190873021479507No Hit
CCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGC359600.10925928373490526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)