FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25893408
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283545910.950505240561613No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3124711.2067588785531824Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG515410.19905066185185047No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT481840.1860859721516766Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG471600.18213129766464115No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)