FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61409019
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT1356065222.08250875982891TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTAT13702372.231328593606096TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTA11433571.861871462235865TruSeq Adapter, Index 7 (97% over 38bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT7584901.2351443034776375Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTA5359890.8728180464827161TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTATG2601560.4236446115512772TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTATGCC2570220.41854112667066057TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTATGCC1531970.2494698702156437TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGGATTGCATCTCGTAT1271630.20707544603505226TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTATG1233980.20094442479206515TruSeq Adapter, Index 7 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTT992260.16158212851437995TruSeq Adapter, Index 7 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT970500.15803867506823388TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATTGCATCTCGTAT800720.13039127037675036TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGGAT770010.12539037628984107TruSeq Adapter, Index 7 (97% over 38bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT752990.12261879643444558Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT719100.11710006310310869TruSeq Adapter, Index 2 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG697270.113545210679884Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTT663250.10800530781968688TruSeq Adapter, Index 7 (97% over 38bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT618430.10070670563879225TruSeq Adapter, Index 2 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)