FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33232647
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTAT16918635.090966723174353TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCGCGTAT2627890.7907555483016445TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTA1169640.35195511209203406TruSeq Adapter, Index 22 (97% over 37bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG769320.23149525224397563Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCGCGTA715810.21539361580195523TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)