FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56277268
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26621574.730430411085343No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2246100.39911319078246654Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1617320.2873842418931921Illumina Single End PCR Primer 1 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)