FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005791440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91668440
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT16391951.7881781341539136TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGGAT5400300.5891122397195807TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTTT3755320.40966334760360273TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGGTT2476470.2701551373624336TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCGCGTAT1700490.18550441133284257TruSeq Adapter, Index 8 (97% over 37bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT1206580.13162436275778228No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG1148720.12531248486392918Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGGT1024090.11171674787964102TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTT928310.10126822273838193TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)