FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48889669
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT36298377.424548118744678TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCGCGTAT5263931.076695773906753TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTA2682030.5485882917309176TruSeq Adapter, Index 1 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCGCGTA1610180.3293497446260068TruSeq Adapter, Index 1 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATG625500.12794114028466833TruSeq Adapter, Index 1 (97% over 37bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT598570.12243281908903902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)