FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005791446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28290491
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT22397167.916850930583TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCGCGTAT2394730.8464787691383653TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTA1735460.6134428702562992TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTTT836980.2958520585591816TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCGCGTA738230.2609463370572112TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTT713840.2523250656908005TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGGAT454720.16073245246963017TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCGCGTT370500.13096273231878514TruSeq Adapter, Index 20 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATG342010.12089221074317869TruSeq Adapter, Index 20 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCC327240.11567137523346627TruSeq Adapter, Index 20 (97% over 35bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT317010.11205531922369252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)