FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005791454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84350399
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTAT23284552.7604552291447964TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCGCGTAT2397060.2841788572926608TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA1377800.16334244014660795TruSeq Adapter, Index 6 (97% over 37bp)
CTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAGGTT1242400.14729035247361424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTTT1152540.1366371722794103TruSeq Adapter, Index 6 (97% over 37bp)
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAG907490.10758573886532534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)