FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005791670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30249472
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3272041.08168499602241TruSeq Adapter, Index 21 (97% over 40bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC430850.1424322381560908No Hit
CTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTC402140.132941163402786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)