FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005791680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36067199
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT35553819.85765764621755TruSeq Adapter, Index 18 (97% over 40bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC2121880.588312943292325No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC1282850.355683289961053No Hit
GCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCC984580.27298488025088946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTAT861160.23876542228854536TruSeq Adapter, Index 18 (97% over 40bp)
CTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTC842390.23356124771430128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACACCTCGTAT482750.13384737750220083TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTAT387660.10748270194200554TruSeq Adapter, Index 18 (97% over 40bp)
GGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGC387250.1073690252464573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)