FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005791685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30188621
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG830420.2750771557269873Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC709820.2351283286507191No Hit
GATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGC504810.16721863512745416No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT504510.16711925993572213No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGG406560.1346732598352207No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA403700.1337258830073755No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCC348080.11530172246026077No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC316800.10494020246900314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)