FastQCFastQC Report
Sun 24 Jul 2022
EGAF00005791698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005791698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33144740
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC7793612.35138667553283TruSeq Adapter, Index 7 (100% over 50bp)
CTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTC1968100.5937895424734061No Hit
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC671020.2024514296989507No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC545220.16449668936911258No Hit
CCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCC399110.12041427991289115No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC356390.1075253569646345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)