FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005797091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005797091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76702896
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1080870.14091645248961657No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1062620.13853714206566595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT83150.035.705186
CCGCGTT95750.030.756047
AGACCGC106750.028.1777634
GACCGCG109400.027.233725
CGTACGG38600.022.159423
CGCGTTC144850.021.1176178
GCGTACG41700.020.1705382
CGCACTA338350.018.1231682-83
CTCGCTA167900.017.2378461
GAGTCTT722500.016.705774
GCGTTCT179650.016.6037549
CGATCGG378400.016.15478770-71
GGAGTCT746000.016.1413923
TCGCTAT179250.016.0919932
CCTACGT376400.015.56551124-25
TACGTTC377450.015.54737926-27
GGAGTGC1064450.015.1403588
CTACGTT376750.014.81352726-27
AGTCTTG846300.014.7837055
GAGTGCA1094250.014.6931879
Produced by FastQC (version 0.11.5)